Anti-MEK2 Antibody (3H388) is a Mouse antibody targeting MEK2. Anti-MEK2 Antibody (3H388) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

3H388

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (ascites of TMAB-08731 4C3) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (ascites of TMAB-08731 4C3) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (ascites of TMAB-08731 4C3) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Sample: HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-MEK2 (TMAB-08731) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD
5. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (TMAB-08731-4C3) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (TMAB-08731) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK2) Monoclonal Antibody, Unconjugated (TMAB-08731) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
8. Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human 293T cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human U937 cell lysates
Lane 5: Human MOLT-4 cell lysates
Primary: Anti-MEK2 (TMAB-08731) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa

WB: 1:1000-2000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:100-500

Monoclonal

Recombinant Protein: human MEK2 Protein

Human

MAP2K2

Dual specificity mitogen-activated protein kinase kinase 2

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.