Anti-MEK1 Polyclonal Antibody is a Rabbit antibody targeting MEK1. Anti-MEK1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Rabbit)

1. Images provided the Independent Validation Program (badge number 029646) Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (TMAB-08729) at 1: 250 overnight at room temperature followed by conjugation to secondary antibody.
2. Tissue/cell: human rectal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-MEK1/MAPKK1/MEKK1 Polyclonal Antibody, Unconjugated (TMAB-08729) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MEK1) Polyclonal Antibody, Unconjugated (TMAB-08729) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human MEK1

Human

MAP2K2

Dual specificity mitogen-activated protein kinase kinase 2

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.