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Anti-MEK1 Antibody (1O963)

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Catalog No. TMAY-03226
Alias Prkmk1, mitogen-activated protein kinase kinase 1, MEKK1, Mek1, MAPKK1

Anti-MEK1 Antibody (1O963) is a Rabbit antibody targeting MEK1. Anti-MEK1 Antibody (1O963) can be used in WB,ICC/IF.

Anti-MEK1 Antibody (1O963)

Anti-MEK1 Antibody (1O963)

😃Good
Catalog No. TMAY-03226Alias Prkmk1, mitogen-activated protein kinase kinase 1, MEKK1, Mek1, MAPKK1
Anti-MEK1 Antibody (1O963) is a Rabbit antibody targeting MEK1. Anti-MEK1 Antibody (1O963) can be used in WB,ICC/IF.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MEK1 Antibody (1O963) is a Rabbit antibody targeting MEK1. Anti-MEK1 Antibody (1O963) can be used in WB,ICC/IF.
AliasPrkmk1, mitogen-activated protein kinase kinase 1, MEKK1, Mek1, MAPKK1
Ig Type
Monoclonal Rabbit IgG
Clone
1O963
Reactivity
Mouse
Verified Activity
1. Anti-MAP2K1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: RAW246.7 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:43 kDa.

-Observed band size:43 kDa.

2. Immunofluorescence staining of MEK1 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse MEK1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Application
WB,ICC/IF
Recommended Dose
WB: 1:500-1:2000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse MEK1/MAP2K1 (rM MEK1/MAP2K1; TMPY-04569; P31938; Met1-Ile393).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundMEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Mouse MEK1/MAP2K1 Protein (TMPY-04569)
Antigen Species
Mouse
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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