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Anti-MEIS2 Antibody (9K20)

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Catalog No. TMAB-08726

Anti-MEIS2 Antibody (9K20) is a Rabbit antibody targeting MEIS2. Anti-MEIS2 Antibody (9K20) can be used in FCM.

Anti-MEIS2 Antibody (9K20)

Anti-MEIS2 Antibody (9K20)

Copy Product Info
😃Good
Catalog No. TMAB-08726
Anti-MEIS2 Antibody (9K20) is a Rabbit antibody targeting MEIS2. Anti-MEIS2 Antibody (9K20) can be used in FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1487-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-MEIS2 Antibody (9K20) is a Rabbit antibody targeting MEIS2. Anti-MEIS2 Antibody (9K20) can be used in FCM.
Ig Type
IgG
Clone
9K20
Reactivity
Human (predicted:Rat,Mouse)
Verified Activity
The HepG2 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-MEIS2 antibody (TMAB-08726): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
FCM: 1μg/Test
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityExpressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHox, Pbx and Meis families of transcription factors form heteromeric complexes and bind DNA through specific homeobox domains. Hox proteins are involved in regulating tissue patterning during development, and are also expressed in lineage- and stage-specific patterns during adult hematopoietic differentiation and in leukemias. The Hox proteins, which include paralog groups 1-10, have a low intrinsic binding affinity for DNA and are instead associated into cooperative DNA binding complexes with Pbx or the Pbx- related Meis proteins, which result in an enhanced Hox-DNA binding affinity and an increased selectivity for the binding site. Both Meis1 and Meis2 (also known as Meis-related gene 1 or Mrg1) are members of the TALE (“three amino acid loop extension”) family of homeodomain-containing proteins. In addition to binding with Hox proteins, Meis1 also forms heterodimers with the ubiquitously expressed Pbx proteins, including Pbx1, Pbx2 and Pbx3, and these complexes contain distinct DNA-binding specificities. Like Hox and Pbx proteins, Meis1 is implicated in oncogenesis, as it is overexpressed as a result of adjacent retroviral insertion in BHX-2 myeloid leukemias. Two Meis-related proteins, Meis2 and Meis3 (also designated Mrg1 and Mrg2, respectively), possess largely similar sequence identity with Meis1 and are expressed in normal tissues and myeloid leukemias. In the pancreas, Meis2 preferentially associates with Pbx1, and together they associate with the pancreas-specific homeodomain factor, Pdx1, to repress Pdx1-induced transcriptional activation.
Antigen Details
Immunogen
A synthesized peptide: human MEIS2
Antigen Species
Human
Gene Name
MEIS2
Gene ID
Protein Name
Homeobox protein Meis2
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 52 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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