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Anti-ME1 Polyclonal Antibody
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Catalog No. TMAB-08697
Anti-ME1 Polyclonal Antibody is a Rabbit antibody targeting ME1. Anti-ME1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-ME1 Polyclonal Antibody
Anti-ME1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08697
Anti-ME1 Polyclonal Antibody is a Rabbit antibody targeting ME1. Anti-ME1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-ME1 Polyclonal Antibody is a Rabbit antibody targeting ME1. Anti-ME1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ME1) Polyclonal Antibody, Unconjugated (TMAB-08697) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Primary: Anti-ME1 (TMAB-08697) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Expressed in all tissues tested including liver, placenta and white adipose tissue. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. |
| Immunogen | KLH conjugated synthetic peptide: human NADP ME |
| Antigen Species | Human |
| Gene Name | ME1 |
| Gene ID | |
| Protein Name | NADP-dependent malic enzyme |
| Uniprot ID |
| Molecular Weight | Theoretical: 64 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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