Anti-MCT1/Malignant T cell amplified sequence 1 Polyclonal Antibody is a Rabbit antibody targeting MCT1/Malignant T cell amplified sequence 1. Anti-MCT1/Malignant T cell amplified sequence 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated (TMAB-08679) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-MCT1 Polyclonal Antibody, Unconjugated (TMAB-08679) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C.
3. Sample:
Brain (Rat) lysate at 45ug;
Colon carcinomA (Human) lysate at 45ug;
Primary: Anti-MCT1 (TMAB-08679) at 1: 200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000;
Predicted band size: 55 kD
Observed band size: 55 kD
4. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Stomach (Mouse) Lysate at 40 μg
Lane 3: Heart (Mouse) Lysate at 40 μg
Lane 4: Liver (Rat) Lysate at 40 μg
Lane 5: Stomach (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Primary: Anti-MCT1 (TMAB-08679) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human MCT1

Human

SLC16A1

Monocarboxylate transporter 1

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.