Anti-MCPIP1 Polyclonal Antibody is a Rabbit antibody targeting MCPIP1. Anti-MCPIP1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Cow,Horse,Rabbit)

Sample:
Lane 1: Mouse Raw264.7 cell lysates
Lane 2: Mouse Raw264.7 (LPS) cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human HepG2 cell lysates
Primary: Anti-MCPIP1 (TMAB-08675) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 60 kDa
2. k control (Black line): Hela (Black).
Primary Antibody (green line): Rabbit Anti-MCPIP1 antibody
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at -20℃. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human MCPIP1

Human

ZC3H12A

Endoribonuclease ZC3H12A

Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19.