Anti-MBP Antibody (3G275) is a Mouse antibody targeting MBP. Anti-MBP Antibody (3G275) can be used in IHC-P,IHC-Fr,IF,WB.

IgG1, k

3G275

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:500 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MBP Monoclonal Antibody, Unconjugated (TMAB-08649) at 1:500 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
9. 25 ug total protein per lane of various lysates (see on figure) probed with MBP monoclonal antibody, unconjugated (TMAB-08649) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000; WB: 1:1000-2000

Monoclonal

KLH conjugated synthetic peptide: human MBP

Human

MBP

Myelin basic protein

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.