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Anti-Mast Cell Tryptase Antibody (3T990)
Catalog No. TMAB-08627 Copy Product Info
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Anti-Mast Cell Tryptase Antibody (3T990) is a Rabbit antibody targeting Mast Cell Tryptase. Anti-Mast Cell Tryptase Antibody (3T990) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Mast Cell Tryptase Antibody (3T990)
Copy Product Info🥰Excellent
Catalog No. TMAB-08627
Anti-Mast Cell Tryptase Antibody (3T990) is a Rabbit antibody targeting Mast Cell Tryptase. Anti-Mast Cell Tryptase Antibody (3T990) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Mast Cell Tryptase Antibody
(3T990)
(3T990)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Mast Cell Tryptase Antibody (3T990) is a Rabbit antibody targeting Mast Cell Tryptase. Anti-Mast Cell Tryptase Antibody (3T990) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 3T990 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (TMAB-08627) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (TMAB-08627) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (TMAB-08627) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (TMAB-08627) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. 25 μg total protein per lane of various lysates (see on figure) probed with Mast Cell Tryptase monoclonal antibody, unconjugated (TMAB-08627) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-5000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Note=Released from the secretory granules upon mast cell activation. |
| Tissue Specificity | Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. This gene was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide: human Tryptase 1 |
| Antigen Species | Human |
| Gene Name | TPSAB1 |
| Gene ID | |
| Protein Name | Tryptase alpha/beta-1 |
| Uniprot ID | |
| Function | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. |
| Molecular Weight | Theoretical: 23 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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