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Anti-MARCH9 Polyclonal Antibody

Catalog No. TMAB-08612 Copy Product Info
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Anti-MARCH9 Polyclonal Antibody is a Rabbit antibody targeting MARCH9. Anti-MARCH9 Polyclonal Antibody can be used in WB,FCM.

Anti-MARCH9 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-08612

Anti-MARCH9 Polyclonal Antibody is a Rabbit antibody targeting MARCH9. Anti-MARCH9 Polyclonal Antibody can be used in WB,FCM.

Anti-MARCH9 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2227-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-MARCH9 Polyclonal Antibody is a Rabbit antibody targeting MARCH9. Anti-MARCH9 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit IgG (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1: 200 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Sample: MCF-7 (Human) Cell Lysate at 40 μg
Primary: Anti-MARCH9 (TMAB-08612) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD
Application
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
Tissue SpecificityUbiquitously expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundUbiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). MARCH9 (membrane-associated ring finger (C3HC4) 9), also known as RNF179, is a 346 amino acid multi-pass membrane protein that localizes to the golgi apparatus and contains one RING-CH-type zinc finger. Expressed ubiquitously, MARCH9 exists as a homodimer and functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to promote the degradation of target proteins, such as CD4 and MHC-I. Multiple isoforms of MARCH9 exist due to alternative splicing events.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MARCH9
Antigen Species
Human
Gene Name
MARCHF9
Gene ID
Protein Name
E3 ubiquitin-protein ligase MARCHF9
Uniprot ID
Function
E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Chemical Properties
Molecular WeightTheoretical: 38 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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