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Anti-MARCH1 Polyclonal Antibody

Catalog No. TMAB-08605 Copy Product Info
🥰Excellent
Anti-MARCH1 Polyclonal Antibody is a Rabbit antibody targeting MARCH1. Anti-MARCH1 Polyclonal Antibody can be used in WB.

Anti-MARCH1 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-08605

Anti-MARCH1 Polyclonal Antibody is a Rabbit antibody targeting MARCH1. Anti-MARCH1 Polyclonal Antibody can be used in WB.

Anti-MARCH1 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-MARCH1 Polyclonal Antibody is a Rabbit antibody targeting MARCH1. Anti-MARCH1 Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Mouse (predicted:Human)
Verified Activity
Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-MARCH1 (TMAB-08605) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 30 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationGolgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein (By similarity). Lysosome membrane; Multi-pass membrane protein (By similarity). Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Tissue SpecificityExpressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B cells, moderate in dendritic cells and low in splenic T cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundE3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, downregulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MARCH1
Antigen Species
Human
Gene Name
MARCHF1
Gene ID
55016
Protein Name
E3 ubiquitin-protein ligase MARCHF1
Uniprot ID
Q8TCQ1
Function
E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Chemical Properties
Molecular WeightTheoretical: 32 kDa. Actual: 30 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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