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Anti-Marapsin Antibody (8L443)
😃Good
Catalog No. TMAY-02243
Alias UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2
Anti-Marapsin Antibody (8L443) is a Rabbit antibody targeting Marapsin. Anti-Marapsin Antibody (8L443) can be used in WB,IP.
Anti-Marapsin Antibody (8L443)
Anti-Marapsin Antibody (8L443)
😃Good
Catalog No. TMAY-02243Alias UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2
Anti-Marapsin Antibody (8L443) is a Rabbit antibody targeting Marapsin. Anti-Marapsin Antibody (8L443) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Marapsin Antibody (8L443) is a Rabbit antibody targeting Marapsin. Anti-Marapsin Antibody (8L443) can be used in WB,IP. |
| Alias | UNQ1884/PRO4327, protease, serine 27, MPN, CAPH2 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8L443 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PRSS27 rabbit monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:32 kDa. -Observed band size:32 kDa. 2. PRSS27 was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -2 µL anti-PRSS27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PRSS27 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 32 kDa. -Observed band size: 32 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Pancreasin / Marapsin / PRSS27 (rh Pancreasin / Marapsin / PRSS27; TMPY-02117; Q9BQR3; Met1-Lys290). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The name "Pancreasin" because it is transcribed strongly in the pancreas. This secreted, tryptic serine protease, also known as Marapsin or PRSS27 (Protease, serine, 27), is a member of the peptidase S1 family. Pancreasin is inhibited by benzamidine and leupeptin but resists several classic inhibitors of trypsin. Marapsin was constitutively expressed in nonkeratinizing stratified squamous epithelia of human esophagus, tonsil, cervix, larynx, and cornea. In fact, marapsin was the second most strongly up-regulated protease in psoriatic lesions, where expression was localized to the upper region of the hyperplastic epidermis. Similarly, in the hyperproliferative epithelium of regenerating murine skin wounds, marapsin localized to the suprabasal layers, where keratinocytes undergo squamous differentiation. Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Pancreasin / Marapsin / PRSS27 protein (TMPY-02117) |
| Antigen Species | Human |
| Biology Area | Serine Proteases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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