Anti-MAPT/Tau Antibody (1F539) is a Mouse antibody targeting MAPT/Tau. Anti-MAPT/Tau Antibody (1F539) can be used in WB,IHC-P,IHC-Fr,IF.

IgG3

1F539

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Human Tau-4 Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
5. 25 ug total protein per lane of various lysates (see on figure) probed with Tau-4 monoclonal antibody, unconjugated (TMAB-08598) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of TMAB-08598) at 1: 1500 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of TMAB-08598) at 1: 1500 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Tau-4 Monoclonal Antibody, Unconjugated (ascites of TMAB-08598) at 1: 1500 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, BF594), DAPI (blue) was used to stain the cell nuclei.
10. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, BF594), DAPI (blue) was used to stain the cell nuclei.
11. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAPT/Tau Monoclonal Antibody, Unconjugated (TMAB-08598) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (Red, BF594), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: Human Tau-4 Protein

Human

MAPT

Microtubule-associated protein tau

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.