Anti-MAPK3 Antibody (1K596) is a Rabbit antibody targeting MAPK3. Anti-MAPK3 Antibody (1K596) can be used in FCM,ICC/IF,IHC,IP,WB.

IgG

1K596

Human,Mouse

1. Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat, Lane 3: K562.
2. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
5. ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining ERK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Jurkat cells with ERK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein