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Anti-MAPK10 Antibody (7M343) is a Rabbit antibody targeting MAPK10. Anti-MAPK10 Antibody (7M343) can be used in WB,ICC/IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-MAPK10 Antibody (7M343) is a Rabbit antibody targeting MAPK10. Anti-MAPK10 Antibody (7M343) can be used in WB,ICC/IF,FCM. |
Synonyms | Stress-activated protein kinase JNK3, Stress-activated protein kinase 1b, SAPK1b, SAPK1B, PRKM10, Mitogen-activated protein kinase 10, MAPK 10, MAP kinase p49 3F12, MAP kinase 10, JNK3A, JNK3, EC 2.7.11.24, c-Jun N-terminal kinase 3 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution. 2. ICC staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining JNK3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Jurkat cells with JNK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500; ICC/IF: 1:100-500; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced). |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 53 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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