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Anti-MAP3K3 Antibody (2Q519)
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Catalog No. TMAC-02557
Alias Mitogen activated protein kinase kinase kinase 3, MEKK3, MEKK 3, MEK kinase 3, MAPKKK3, MAPK/ERK kinase kinase 3, MAP/ERK kinase kinase 3, M3K3_HUMAN
Anti-MAP3K3 Antibody (2Q519) is a Rabbit antibody targeting MAP3K3. Anti-MAP3K3 Antibody (2Q519) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-MAP3K3 Antibody (2Q519)
Anti-MAP3K3 Antibody (2Q519)
😃Good
Catalog No. TMAC-02557Alias Mitogen activated protein kinase kinase kinase 3, MEKK3, MEKK 3, MEK kinase 3, MAPKKK3, MAPK/ERK kinase kinase 3, MAP/ERK kinase kinase 3, M3K3_HUMAN
Anti-MAP3K3 Antibody (2Q519) is a Rabbit antibody targeting MAP3K3. Anti-MAP3K3 Antibody (2Q519) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-MAP3K3 Antibody (2Q519) is a Rabbit antibody targeting MAP3K3. Anti-MAP3K3 Antibody (2Q519) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Mitogen activated protein kinase kinase kinase 3, MEKK3, MEKK 3, MEK kinase 3, MAPKKK3, MAPK/ERK kinase kinase 3, MAP/ERK kinase kinase 3, M3K3_HUMAN |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293T, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin. 4. ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-κB activation in response to a variety of agonists including TNFα, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKβ/NF-κB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3β binding and inhibition of the kinase activity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 71 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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