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Anti-MAP3K14 Polyclonal Antibody

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Catalog No. TMAB-01101

Anti-MAP3K14 Polyclonal Antibody is a Rabbit antibody targeting MAP3K14. Anti-MAP3K14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-MAP3K14 Polyclonal Antibody

Anti-MAP3K14 Polyclonal Antibody

😃Good
Catalog No. TMAB-01101
Anti-MAP3K14 Polyclonal Antibody is a Rabbit antibody targeting MAP3K14. Anti-MAP3K14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MAP3K14 Polyclonal Antibody is a Rabbit antibody targeting MAP3K14. Anti-MAP3K14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Kidney (Mouse) lysate at 30 μg;
Brain (Mouse) lysate at 30 μg
Primary: Anti-NFkB Inducing Kinase NIK (TMAB-01101) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 104 kDa
Observed band size: 104 kDa
2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSNIK Polyclonal Antibody, Unconjugated (TMAB-01101) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFkB Inducing Kinase NIK antibody (TMAB-01101)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NIK
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Kinases,NFkB pathway,Other Kinases,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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