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Anti-MAP3K14 Polyclonal Antibody is a Rabbit antibody targeting MAP3K14. Anti-MAP3K14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-MAP3K14 Polyclonal Antibody is a Rabbit antibody targeting MAP3K14. Anti-MAP3K14 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: Kidney (Mouse) lysate at 30 μg; Brain (Mouse) lysate at 30 μg Primary: Anti-NFkB Inducing Kinase NIK (TMAB-01101) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution; Predicted band size: 104 kDa Observed band size: 104 kDa 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HSNIK Polyclonal Antibody, Unconjugated (TMAB-01101) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFkB Inducing Kinase NIK antibody (TMAB-01101) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq]. |
Immunogen | KLH conjugated synthetic peptide: human NIK |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Kinases,NFkB pathway,Other Kinases,NFkB Pathway |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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