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Anti-MAP2K3 Antibody (7F635)
😃Good
Catalog No. TMAC-02546
Alias zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3
Anti-MAP2K3 Antibody (7F635) is a Rabbit antibody targeting MAP2K3. Anti-MAP2K3 Antibody (7F635) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-MAP2K3 Antibody (7F635)
Anti-MAP2K3 Antibody (7F635)
😃Good
Catalog No. TMAC-02546Alias zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3
Anti-MAP2K3 Antibody (7F635) is a Rabbit antibody targeting MAP2K3. Anti-MAP2K3 Antibody (7F635) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-MAP2K3 Antibody (7F635) is a Rabbit antibody targeting MAP2K3. Anti-MAP2K3 Antibody (7F635) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | zMKK 3, SKK2, Protein kinase mitogen activated kinase 3, PRKMK3, PRKMK 3, MPK 3, MP2K6, MP2K3, mMKK3b, mMKK 3b, MKK3, MKK 3, Mitogen activated protein kinase kinase 3, MEK3, MEK 3, MAPKK3, MAPKK 3, MAPK kinase 3, MAPK ERK kinase 3, MAP2K 3, MAP Kinase Kinase 3, MAP kinase kinase 3, ERK kinase 3, Dual specificity mitogen activated protein kinase kinase 3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MEK3+MEK6 on different lysates using anti-MEK3+MEK6 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK3+MEK6 antibody. Counter stained with hematoxylin. 3. ICC staining MEK3+MEK6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining MEK3+MEK6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining MEK3+MEK6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 39/37 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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