Anti-MAP2K1/2 Antibody (7V534) is a Rabbit antibody targeting MAP2K1/2. Anti-MAP2K1/2 Antibody (7V534) can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF.

IgG

7V534

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2 k1/2 Monoclonal Antibody, Unconjugated (TMAB-08564) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2 k1/2 Monoclonal Antibody, Unconjugated (TMAB-08564) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2 k1/2 Monoclonal Antibody, Unconjugated (TMAB-08564) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2 k1/2 Monoclonal Antibody, Unconjugated (TMAB-08564) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2 k1/2 Monoclonal Antibody, Unconjugated (TMAB-08564) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with MAP2 k1/2 monoclonal antibody, unconjugated (TMAB-08564) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human MEK1

Human

Map2k1

Dual specificity mitogen-activated protein kinase kinase 1