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Anti-MAP2 Antibody (1T377)

😃Good
Catalog No. TMAB-01099

Anti-MAP2 Antibody (1T377) is a Mouse antibody targeting MAP2. Anti-MAP2 Antibody (1T377) can be used in IHC-P,IHC-Fr,IF.

Anti-MAP2 Antibody (1T377)

Anti-MAP2 Antibody (1T377)

😃Good
Catalog No. TMAB-01099
Anti-MAP2 Antibody (1T377) is a Mouse antibody targeting MAP2. Anti-MAP2 Antibody (1T377) can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MAP2 Antibody (1T377) is a Mouse antibody targeting MAP2. Anti-MAP2 Antibody (1T377) can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG1
Clone
1T377
Reactivity
Human
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAP2) Monoclonal Antibody, Unconjugated (TMAB-01099) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm, cytoskeleton (Probable).
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
Antigen Details
Immunogen
Recombinant Protein: human MAP2 protein
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Dendrite marker,Soma marker,map2,MAP,Neuron Restricted Lineage
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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