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Anti-MAP1D Polyclonal Antibody

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Catalog No. TMAB-08557

Anti-MAP1D Polyclonal Antibody is a Rabbit antibody targeting MAP1D. Anti-MAP1D Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MAP1D Polyclonal Antibody

Anti-MAP1D Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-08557
Anti-MAP1D Polyclonal Antibody is a Rabbit antibody targeting MAP1D. Anti-MAP1D Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-MAP1D Polyclonal Antibody is a Rabbit antibody targeting MAP1D. Anti-MAP1D Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Dog,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample: SW480 (Human) Cell Lysate at 30 μg
Primary: Anti-MAP1D (TMAB-08557) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
2. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAP1D) Polyclonal Antibody, Unconjugated (TMAB-08557) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion.
Tissue SpecificityOverexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level).
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MAP1D
Antigen Species
Human
Gene Name
METAP1D
Gene ID
254042
Protein Name
Methionine aminopeptidase 1D, mitochondrial
Uniprot ID
Q6UB28
Function
Removes the amino-terminal methionine from nascent proteins (By similarity). May play a role in colon tumorigenesis.
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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