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Anti-MAGEB3 Polyclonal Antibody
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Catalog No. TMAB-08522
Anti-MAGEB3 Polyclonal Antibody is a Rabbit antibody targeting MAGEB3. Anti-MAGEB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MAGEB3 Polyclonal Antibody
Anti-MAGEB3 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08522
Anti-MAGEB3 Polyclonal Antibody is a Rabbit antibody targeting MAGEB3. Anti-MAGEB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-MAGEB3 Polyclonal Antibody is a Rabbit antibody targeting MAGEB3. Anti-MAGEB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Horse) |
| Verified Activity | 1. Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (TMAB-08522) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: Human melanoma tumor; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (TMAB-08522) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Tissue/cell: mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-MAGEB3 Polyclonal Antibody, Unconjugated (TMAB-08522) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 4. Sample: Brain (Mouse) Lysate at 40 μg Primary: Anti-MAGEB3 (TMAB-08522) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Expressed in testis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | MAGEB3 is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is localized in the DSS (dosage-sensitive sex reversal) critical region. It is expressed in testis and placenta, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. |
| Immunogen | KLH conjugated synthetic peptide: human MAGEB3 |
| Antigen Species | Human |
| Gene Name | MAGEB3 |
| Gene ID | |
| Protein Name | Melanoma-associated antigen B3 |
| Uniprot ID |
| Molecular Weight | Theoretical: 39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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