Anti-MAGEA11 Polyclonal Antibody is a Rabbit antibody targeting MAGEA11. Anti-MAGEA11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human

1. Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U-2 OS cell lysates
Primary: Anti-MAGEA11 (TMAB-08516) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 53 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (MAGEA11) Polyclonal Antibody, Unconjugated (TMAB-08516) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human MAGEA11

Human

MAGEA11

Melanoma-associated antigen 11

Acts as androgen receptor co-regulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation or aspects of tumor progression.