Anti-MAG Polyclonal Antibody is a Rabbit antibody targeting MAG. Anti-MAG Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat)

1. Sample: U251 Cell Lysate at 30 μg
Primary: Anti-MAG (TMAB-08509) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 67 kD
Observed band size: 67 kD
2. Blank control: sH-SY5Y.
Primary Antibody (green line): Rabbit Anti-MAG antibody (TMAB-08509)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human MAG-a/b

Human

MAG

Myelin-associated glycoprotein

Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.