Anti-Mafa Polyclonal Antibody is a Rabbit antibody targeting Mafa. Anti-Mafa Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Rat (predicted:Human,Mouse)

1. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Mafa Polyclonal Antibody, Unconjugated (TMAB-01095) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Rat Pancreas tissue lysates
Primary: Anti-Mafa (TMAB-01095) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 47 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse Mafa

Mouse

MAFA

Transcription factor MafA

Co-factors,Other factors,Insulin / Insulin-like

Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Regulates the insulin gene transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.