Anti-MAdCAM-1 Polyclonal Antibody is a Rabbit antibody targeting MAdCAM-1. Anti-MAdCAM-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat)

1. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-MAdCAM-1 Polyclonal Antibody, Unconjugated (TMAB-08503) 1: 200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lymph node (Mouse) Lysate at 30 μg
Spleen (Mouse) Lysate at 30 μg
Primary: Anti-MAdCAM-1 (TMAB-08503) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 50 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: rat MAdCAM-1

Rat

MADCAM1

Mucosal addressin cell adhesion molecule 1

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.