Anti-MAD3 Antibody (9E238) is a Rabbit antibody targeting MAD3. Anti-MAD3 Antibody (9E238) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

9E238

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with MAD3 monoclonal antibody, unconjugated (TMAB-08501) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (MAD3) monoclonal Antibody, unconjugated (TMAB-08501) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:50-200; IHC-Fr=1:50-200; IHC-P=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human MAD3

Human

MXD3

Max dimerization protein 3

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.