Anti-MAD1 Antibody (5Y280) is a Rabbit antibody targeting MAD1. Anti-MAD1 Antibody (5Y280) can be used in FCM, WB.

IgG

5Y280

Human,Mouse,Rat

1. Western blot analysis of A431 cell lysate. Using MAD1 (TMAB-08496) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-MAD1 antibody (TMAB-08496, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:1000-2000

Monoclonal

A synthesized peptide: human MAD1

Human

MAD1L1

Mitotic spindle assembly checkpoint protein MAD1

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.