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Anti-MAD1 Antibody (5Y280)
😃Good
Catalog No. TMAB-08496
Anti-MAD1 Antibody (5Y280) is a Rabbit antibody targeting MAD1. Anti-MAD1 Antibody (5Y280) can be used in FCM, WB.
Anti-MAD1 Antibody (5Y280)
Anti-MAD1 Antibody (5Y280)
😃Good
Catalog No. TMAB-08496
Anti-MAD1 Antibody (5Y280) is a Rabbit antibody targeting MAD1. Anti-MAD1 Antibody (5Y280) can be used in FCM, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MAD1 Antibody (5Y280) is a Rabbit antibody targeting MAD1. Anti-MAD1 Antibody (5Y280) can be used in FCM, WB. |
| Ig Type | IgG |
| Clone | 5Y280 |
| Reactivity | Human,Mouse,Rat |
| Application | |
| Recommended Dose | FCM=1:50-100; WB=1:1000-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Co-localizes with NEK2 at the kinetochore. |
| Tissue Specificity | Expressed weakly at G0/G1 and highly at late S and G2/M phase. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate.Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1. |
| Immunogen | A synthesized peptide: human MAD1 |
| Antigen Species | Human |
| Gene Name | MAD1L1 |
| Gene ID | |
| Protein Name | Mitotic spindle assembly checkpoint protein MAD1 |
| Uniprot ID | |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. |
| Molecular Weight | Theoretical: 83 kDa. Actual: 80 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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