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Anti-macroH2A.1 Antibody (7A412)
Catalog No. TMAC-02442 Copy Product Info
🥰Excellent
Anti-macroH2A.1 Antibody (7A412) is a Rabbit antibody targeting macroH2A.1. Anti-macroH2A.1 Antibody (7A412) can be used in ICC/IF,IHC,WB.
Anti-macroH2A.1 Antibody (7A412)
Copy Product Info🥰Excellent
Catalog No. TMAC-02442
Anti-macroH2A.1 Antibody (7A412) is a Rabbit antibody targeting macroH2A.1. Anti-macroH2A.1 Antibody (7A412) can be used in ICC/IF,IHC,WB.
Anti-macroH2A.1 Antibody
(7A412)
(7A412)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
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| Description | Anti-macroH2A.1 Antibody (7A412) is a Rabbit antibody targeting macroH2A.1. Anti-macroH2A.1 Antibody (7A412) can be used in ICC/IF,IHC,WB. |
| Ig Type | IgG |
| Clone | 7A412 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution. Positive control: Lane 1: Hela, Lane 2: Mouse placenta tissue, Lane 3: MCF-7. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin. 6. ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.         |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 40 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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