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Anti-LY75 Antibody (3M662)

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Catalog No. TMAB-08466

Anti-LY75 Antibody (3M662) is a Rabbit antibody targeting LY75. Anti-LY75 Antibody (3M662) can be used in IHC-P,IHC-Fr,IF,WB.

Anti-LY75 Antibody (3M662)

Anti-LY75 Antibody (3M662)

Copy Product Info
😃Good
Catalog No. TMAB-08466
Anti-LY75 Antibody (3M662) is a Rabbit antibody targeting LY75. Anti-LY75 Antibody (3M662) can be used in IHC-P,IHC-Fr,IF,WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-LY75 Antibody (3M662) is a Rabbit antibody targeting LY75. Anti-LY75 Antibody (3M662) can be used in IHC-P,IHC-Fr,IF,WB.
Ig Type
IgG
Clone
3M662
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with LY75 monoclonal antibody, unconjugated (TMAB-08466) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated (TMAB-08466) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated (TMAB-08466) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated (TMAB-08466) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated (TMAB-08466) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LY75 Monoclonal Antibody, Unconjugated (TMAB-08466) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
Tissue SpecificityExpressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundPredicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome. [provided by Alliance of Genome Resources, Apr 2022]
Antigen Details
Immunogen
A synthesized peptide: human DEC 205
Gene Name
LY75
Gene ID
Protein Name
Lymphocyte antigen 75
Function
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B-lymphocytes.
Chemical Properties
Molecular WeightTheoretical: 195 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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