Anti-Ly6A Antibody (2H276) is a Rabbit antibody targeting Ly6A. Anti-Ly6A Antibody (2H276) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2H276

Mouse

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: Mouse thymus, 2: C2C12
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 13 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1: 2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: C2C12 cell lysate, the Ly6A Rabbit mAb pre-adsorbed with 3 μM of the synthetic peptides; 2: C2C12 cell lysate, the Ly6A Rabbit mAb with no peptide blocking.
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 14 kDa
Observed MW: 13 kDa
3. Tissue: Mouse testis
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-08461

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Ly6a

Lymphocyte antigen 6A-2/6E-1

T-cell activation.