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Anti-LW1/HSFX1 Polyclonal Antibody

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Catalog No. TMAB-08454

Anti-LW1/HSFX1 Polyclonal Antibody is a Rabbit antibody targeting LW1/HSFX1. Anti-LW1/HSFX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-LW1/HSFX1 Polyclonal Antibody

Anti-LW1/HSFX1 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-08454
Anti-LW1/HSFX1 Polyclonal Antibody is a Rabbit antibody targeting LW1/HSFX1. Anti-LW1/HSFX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-LW1/HSFX1 Polyclonal Antibody is a Rabbit antibody targeting LW1/HSFX1. Anti-LW1/HSFX1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (TMAB-08454) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Jurkat Cell (Human) Lysate at 40 μg
Hela Cell (Human) Lysate at 40 μg
Primary: Anti-LW1/HSFX1 (TMAB-08454) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus (Potential). Cytoplasm.
Tissue SpecificityTestis-specific.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHSFX1 (heat shock transcription factor family, X linked 1), also known as LW-1, is a 423 amino acid protein that is predominately expressed in testis. Localized to the cytoplasm, HSFX1 is thought to be involved in spermatogenesis and male fertility.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LW1/HSFX1
Antigen Species
Human
Gene Name
HSFX1
Gene ID
Protein Name
Heat shock transcription factor, X-linked
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 45 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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