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Anti-Lumican Antibody (9X464)

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Catalog No. TMAC-02432
Alias Lumican, KSPG lumican, Keratan sulfate proteoglycan lumican

Anti-Lumican Antibody (9X464) is a Rabbit antibody targeting Lumican. Anti-Lumican Antibody (9X464) can be used in FCM,ICC,IHC,WB.

Anti-Lumican Antibody (9X464)

Anti-Lumican Antibody (9X464)

😃Good
Catalog No. TMAC-02432Alias Lumican, KSPG lumican, Keratan sulfate proteoglycan lumican
Anti-Lumican Antibody (9X464) is a Rabbit antibody targeting Lumican. Anti-Lumican Antibody (9X464) can be used in FCM,ICC,IHC,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Lumican Antibody (9X464) is a Rabbit antibody targeting Lumican. Anti-Lumican Antibody (9X464) can be used in FCM,ICC,IHC,WB.
SynonymsLumican, KSPG lumican, Keratan sulfate proteoglycan lumican
Ig Type
IgG
Clone
9X464
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: 293T, Lane 3: Human skin.
2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lumican antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Lumican antibody. Counter stained with hematoxylin.
4. ICC staining Lumican in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Lumican in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Lumican in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of 293T cells with Lumican antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundLumican, a member of the small leucine-rich proteoglycans (SLRPs) family of proteins, is a keratan sulfate proteoglycan present in large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. During collagen fibrillogenesis, lumican regulates the assembly of collagens into higher order fibrils, limiting the growth of fibrils in diameter. Lumican is present in the extracellular matrix of human articular cartilage at all ages, with a far greater abundance in the adult. The gene encoding human lumican maps to chromosome 12q21.3-q22.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: human Lumican aa 50-250
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 38 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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