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Anti-LTF/Lactoferrin Polyclonal Antibody is a Rabbit antibody targeting LTF/Lactoferrin. Anti-LTF/Lactoferrin Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-LTF/Lactoferrin Polyclonal Antibody is a Rabbit antibody targeting LTF/Lactoferrin. Anti-LTF/Lactoferrin Polyclonal Antibody can be used in WB,FCM. |
Ig Type | IgG |
Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Sheep) |
Verified Activity | 1. Sample: Breast milk (Human) Lysate at 40 μg Primary: Anti-Lactoferrin (TMAB-01086) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa 2. Blank control: U-937. Primary Antibody (green line): Rabbit Anti-Lactoferrin antibody (TMAB-01086) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. U-937 cells were fixed with 4% PFA for 10 min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody (TMAB-01086) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 1ug/test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Isoform 1: Secreted. Isoform DeltaLf: Cytoplasm. Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. |
Immunogen | KLH conjugated synthetic peptide: human Lactoferrin |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Apoptosis,Apoptosis,Serum Proteins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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