Anti-LTBR Polyclonal Antibody is a Rabbit antibody targeting LTBR. Anti-LTBR Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow)

1. Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Rat Lymph node tissue lysates
Lane 3: Human A431 cell lysates
Primary: Anti-LTBR (TMAB-08445) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa
2. Sample:
HepG2 cell (human) Lysate at 40 μg
Cerebrum (mouse) Lysate at 40 μg
Primary: Anti-LTBR (TMAB-08445) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 4 4 kD
Observed band size: 54 kD
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LTBR) Polyclonal Antibody, Unconjugated (TMAB-08445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human LTBR

Human

LTBR

Tumor necrosis factor receptor superfamily member 3

Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.