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Anti-LTA4H Polyclonal Antibody

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Catalog No. TMAB-08440

Anti-LTA4H Polyclonal Antibody is a Rabbit antibody targeting LTA4H. Anti-LTA4H Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-LTA4H Polyclonal Antibody

Anti-LTA4H Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-08440
Anti-LTA4H Polyclonal Antibody is a Rabbit antibody targeting LTA4H. Anti-LTA4H Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-LTA4H Polyclonal Antibody is a Rabbit antibody targeting LTA4H. Anti-LTA4H Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LTA4H) Polyclonal Antibody, Unconjugated (TMAB-08440) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-LTA4H (TMAB-08440) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 67, 69 kDa
Application
WB
IHC-P
IHC-Fr
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
Tissue SpecificityIsoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundLeukotrienes are a group of bioactive compounds which play important roles in immediate hyposensitivity reactions and inflammation. Leukotriene A4 hydrolase hydrolyzes an epoxide moiety of leukotriene A4 (LTA4) to form leukotriene B4 (LTB4). The enzyme also has some peptidase activity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LTA4H
Antigen Species
Human
Gene Name
LTA4H
Gene ID
4048
Protein Name
Leukotriene A-4 hydrolase
Uniprot ID
P09960
Function
Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity.
Chemical Properties
Molecular WeightTheoretical: 69 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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