Anti-LRP/MVP Polyclonal Antibody is a Rabbit antibody targeting LRP/MVP. Anti-LRP/MVP Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LRP/MVP) Polyclonal Antibody, Unconjugated (TMAB-08393) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. The figure annotation:
The blue histogram is unstained cells.
The Orange histogram is cells stained with Rabbit IgG/FITC
The green histogram is cells stained with Rabbit Anti-LRP/MVP/FITC Conjugated antibody (TMAB-08393-FITC).
Controls
Positive control: HepG 2 cells
Isotype control: Cell lines treated with Rabbit IgG/FITC instead of the primary antibody to confirm that primary antibody binding is specific. 2 μg in 1 00 μL 1 X PBS containing 0.5% BSA.
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (TMAB-08393) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human LRP

Human

MVP

Major vault protein

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.