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Anti-LRCH1 Polyclonal Antibody
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Catalog No. TMAB-08381
Anti-LRCH1 Polyclonal Antibody is a Rabbit antibody targeting LRCH1. Anti-LRCH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-LRCH1 Polyclonal Antibody
Anti-LRCH1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08381
Anti-LRCH1 Polyclonal Antibody is a Rabbit antibody targeting LRCH1. Anti-LRCH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-LRCH1 Polyclonal Antibody is a Rabbit antibody targeting LRCH1. Anti-LRCH1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Spleen (Mouse) Lysate at 40 μg Primary: Anti-LRCH1 (TMAB-08381) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD 2. Sample: Cerebellum (Mouse) Lysate at 40 μg Primary: Anti-LRCH1 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD 3. Sample: U251 Cell (Human) Lysate at 30 μg Primary: Anti-LRCH1 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (TMAB-08381) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (TMAB-08381) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (TMAB-08381) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LRCH1 Polyclonal Antibody, Unconjugated (TMAB-08381) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. The gene encoding LRCH1, also known as Neuronal protein 81 (NP81), has been suggested to be a possible gene locus for susceptibility to osetoarthritis, especially in women, though studies have shown conflicting results. LRCH1 is a 728 amino acid protein that is expressed at highest levels in cerebellum, with lower levels of expression in spinal cord, ovary, kidney and amygdala. In pigs, the gene encoding LRCH1 is one of 22 genes that is associated with leg and body conformation traits that affect productivity and health. There are two isoforms of LRCH1 that are expressed as a result of alternative splicing events. |
| Immunogen | KLH conjugated synthetic peptide: human LRCH1 |
| Antigen Species | Human |
| Gene Name | LRCH1 |
| Gene ID | |
| Protein Name | Leucine-rich repeat and calponin homology domain-containing protein 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 81 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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