Anti-LPLUNC1 Polyclonal Antibody is a Rabbit antibody targeting LPLUNC1. Anti-LPLUNC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane1: Lung (Rat) Lysate at 30 μg
Lane2: Colon carcinomA (Human) Lysate at 30 μg
Primary: Anti-LPLUNC1 (TMAB-08375) at 1: 200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 50 kD
Observed band size: 53 kD
2. Tissue/cell: human Nasal polyps tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated (TMAB-08375) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Sample: JurkaT (Human) Cell Lysate at 30 μg
Primary: Anti-LPLUNC1 (TMAB-08375) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
4. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-LPLUNC1 (TMAB-08375) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human LPLUNC1

Human

BPIFB1

BPI fold-containing family B member 1

May play a role in innate immunity in mouth, nose and lungs.