Anti-LPCAT1 Antibody (9Q186) is a Mouse antibody targeting LPCAT1. Anti-LPCAT1 Antibody (9Q186) can be used in WB.

IgG2b/Kappa

9Q186

Human,Mouse

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 4℃ overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: Mouse spleen
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 55 kDa
Observed MW: 55 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: A431, A549
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 55 kDa
Observed MW: 55 kDa

WB: 1:500-1000

Monoclonal

Lpcat1

Lysophosphatidylcholine acyltransferase 1

Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.