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Anti-LNX2 Polyclonal Antibody
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Catalog No. TMAB-08351 Copy Product Info
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Anti-LNX2 Polyclonal Antibody is a Rabbit antibody targeting LNX2. Anti-LNX2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-LNX2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $222 | 7-10 days | 7-10 days | |
| 100 µL | $372 | 7-10 days | 7-10 days | |
| 200 µL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-LNX2 Polyclonal Antibody is a Rabbit antibody targeting LNX2. Anti-LNX2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Horse,Rabbit) |
| Verified Activity | 1. Sample: U251 (Human) Cell Lysate at 30 μg Primary: Anti-LNX2 (TMAB-08351) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 76 kD 2. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LNX2) Polyclonal Antibody, Unconjugated (TMAB-08351) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | LNX2 (LIGAND OF NUMB PROTEIN X2, PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 1, PDZRN1), which interacts with mammalian Numb and NumbL, contains a RING finger domain, followed by a motif similar to a PTB-binding motif and 4 PDZ domains. Lnx2 and Lnx1 (a proposed relative) are multimodular proteins that bind Numb, a cell fate determinant, through their NPXY motifs. Studies may suggest that Lnx proteins act as molecular scaffolds that promote the aggregation of unrelated, interacting proteins, such as Numb, to definitive subcellular sites. The LNX2 gene maps to chromosome 13q12.2 based on an alignment of the LNX2 sequence with the genomic sequence. Lnx proteins may form large networks by homomeric binding, and their expression patterns overlap with those of the Numb proteins. Furthermore, studies also suggest that the oligomerization of Lnx2 and Numb binding occurs simultaneously. |
| Immunogen | KLH conjugated synthetic peptide: human LNX2 |
| Antigen Species | Human |
| Gene Name | LNX2 |
| Gene ID | |
| Protein Name | Ligand of Numb protein X 2 |
| Uniprot ID |
| Molecular Weight | Theoretical: 76 kDa. Actual: 76 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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