Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (TMAB-01074) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Lipoprotein lipase) Polyclonal Antibody, Unconjugated (TMAB-01074) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Blank control (blue line): raji (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37°C and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-Lipoprotein lipase antibody (TMAB-01074), Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
4. Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-Lipoprotein lipase (TMAB-01074) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human LPL protein

Human

4023

Metabolism of lipids and lipoproteins,Lipases,Lipases,Lipid metabolism,Cancer,Obesity,Lipid metabolism,Adipogenesis