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Anti-LILRB3 Antibody (6S32) is a Rabbit antibody targeting LILRB3. Anti-LILRB3 Antibody (6S32) can be used in FCM,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | 254 € | 7-10 days |
Description | Anti-LILRB3 Antibody (6S32) is a Rabbit antibody targeting LILRB3. Anti-LILRB3 Antibody (6S32) can be used in FCM,ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
Clone | 6S32 |
Reactivity | Mouse |
Verified Activity | 1. Immunofluorescence staining of mouse LILRB3 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse LILRB3 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). 2. Flow cytometric analysis of Mouse LILRB3(CD85a) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse LILRB3(CD85a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
Application | FCM,ICC/IF |
Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LILRB3 (rM LILRB3; TMPY-02633; AAC53219.1; Met1-Tyr640). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Leukocyte immunoglobulin-like receptor subfamily B member 3, also known as Leukocyte immunoglobulin-like receptor 3, Immunoglobulin-like transcript 5, Monocyte inhibitory receptor HL9, CD85 antigen-like family member A, CD85a and LILRB3, is a single-pass type I membrane protein that belongs to the leukocyte receptor cluster (LRC) present on 19q13.4. LILRB3 / CD85a contains four Ig-like C2-type (immunoglobulin-like) domains. LILRB3 / CD85a contains three copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in the modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. LILRB3 / CD85a is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found. |
Conjucates | Unconjugated |
Immunogen | Recombinant Mouse LILRB3 Protein (TMPY-02633) |
Antigen Species | Mouse |
Biology Area | ITIM/ITAM Immunoreceptors and Related Molecules |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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