Anti-LILRB1 Antibody (9N307) is a Rabbit antibody targeting LILRB1. Anti-LILRB1 Antibody (9N307) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

9N307

Human

1. The THP-1 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-LILRB1 antibody (TMAB-08305, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed THP-1 (H) cell; Antibody incubation with (LILRB1) monoclonal Antibody, unconjugated (TMAB-08305) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LILRB1 Monoclonal Antibody, Unconjugated (TMAB-08305) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. 25 ug total protein per lane of various lysates (see on figure) probed with LILRB1 monoclonal antibody, unconjugated (TMAB-08305) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100

Monoclonal

KLH conjugated synthetic peptide: human LILRB1

Human

LILRB1

Leukocyte immunoglobulin-like receptor subfamily B member 1