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Anti-Leucyl tRNA synthetase Polyclonal Antibody

Catalog No. TMAB-08283 Copy Product Info
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Anti-Leucyl tRNA synthetase Polyclonal Antibody is a Rabbit antibody targeting Leucyl tRNA synthetase. Anti-Leucyl tRNA synthetase Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Leucyl tRNA synthetase Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-08283

Anti-Leucyl tRNA synthetase Polyclonal Antibody is a Rabbit antibody targeting Leucyl tRNA synthetase. Anti-Leucyl tRNA synthetase Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Leucyl tRNA synthetase Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Leucyl tRNA synthetase Polyclonal Antibody is a Rabbit antibody targeting Leucyl tRNA synthetase. Anti-Leucyl tRNA synthetase Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (TMAB-08283) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human colorectal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (TMAB-08283) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasmic
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Leucyl tRNA synthetase
Antigen Species
Human
Gene Name
LARS1
Gene ID
Protein Name
Leucine--tRNA ligase, cytoplasmic
Uniprot ID
Function
Leucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of leucine with leucyl tRNA.
Chemical Properties
Molecular WeightTheoretical: 135 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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