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Anti-Leptin Polyclonal Antibody
😃Good
Catalog No. TMAB-01059
Alias obese, ob, leptin
Anti-Leptin Polyclonal Antibody is a Rabbit antibody targeting Leptin. Anti-Leptin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Leptin Polyclonal Antibody
Anti-Leptin Polyclonal Antibody
😃Good
Catalog No. TMAB-01059Alias obese, ob, leptin
Anti-Leptin Polyclonal Antibody is a Rabbit antibody targeting Leptin. Anti-Leptin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Leptin Polyclonal Antibody is a Rabbit antibody targeting Leptin. Anti-Leptin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | obese, ob, leptin |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Polyclonal Antibody, Unconjugated (TMAB-01059) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function. |
| Immunogen | KLH conjugated synthetic peptide: human Leptin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Adipogenesis,Hormones,Metabolism,Obesity,Heart disease,Cancer,Diabetes associated |
| Molecular Weight | Theoretical: 16 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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