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Anti-Leptin Antibody (6V739)
😃Good
Catalog No. TMAY-00488
Alias obese, ob, leptin
Anti-Leptin Antibody (6V739) is a Rabbit antibody targeting Leptin. Anti-Leptin Antibody (6V739) can be used in FCM,ICC/IF.
Anti-Leptin Antibody (6V739)
Anti-Leptin Antibody (6V739)
😃Good
Catalog No. TMAY-00488Alias obese, ob, leptin
Anti-Leptin Antibody (6V739) is a Rabbit antibody targeting Leptin. Anti-Leptin Antibody (6V739) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | 255 € | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Leptin Antibody (6V739) is a Rabbit antibody targeting Leptin. Anti-Leptin Antibody (6V739) can be used in FCM,ICC/IF. |
| Alias | obese, ob, leptin |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6V739 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human LEP expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human LEP monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm . |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Leptin / LEP (rh Leptin / LEP; TMPY-00875; NP_000221.1; Val22-Cys167). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Leptin is one of the most important hormones secreted by adipocytes, as an adipokine that modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. The circulating leptin levels serve as a gauge of energy stores, thereby directing the regulation of energy homeostasis, neuroendocrine function, and metabolism. Recent studies suggest that leptin is physiologically more important as an indicator of energy deficiency, rather than energy excess, and may mediate adaptation by driving increased food intake and directing neuroendocrine function to converse energy, such as inducing hypothalamic hypogonadism to prevent fertilization. One of these functions is the connection between nutritional status and immune competence. The adipocyte-derived hormone Leptin has been shown to regulate the immune response, innate, and adaptive response, both in normal and pathological conditions. Thus, Leptin is a mediator of the inflammatory response. Leptin has a dual effect on bone, acting by two independent mechanisms. As a signal molecule with growth factor characteristics, leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis. However, as a molecule that stimulates sympathetic neurons in the hypothalamus, leptin indirectly inhibits bone formation. This inhibitory effect of leptin mediated by activation of the sympathetic nervous system can be abrogated by the application of blood pressure-reducing beta-blockers, which also inhibit receptors of hypothalamic adrenergic neurons. Leptin appears to regulate some features defining Alzheimer's disease (AD) at the molecular and physiological level. Leptin can stimulate mitogenic and angiogenic processes in peripheral organs. Because leptin levels are elevated in obese individuals and excess body weight has been shown to increase breast cancer risk in postmenopausal women. Furthermore, a recent report clearly shows that targeting leptin signaling may reduce mammary carcinogenesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Leptin / LEP Protein (TMPY-00875) |
| Antigen Species | Human |
| Biology Area | Neurotransmitter Associated Enzymes |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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