Anti-Leptin Antibody (3D628) is a Mouse antibody targeting Leptin. Anti-Leptin Antibody (3D628) can be used in IHC-P,IHC-Fr,IF.

IgG

3D628

Rat (predicted:Human,Mouse)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of TMAB-08274 3C2) at overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of TMAB-08274 3C2) at overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500

Monoclonal

Recombinant Protein: human Leptin

Human

LEP

Leptin

May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.