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Anti-LC3B Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01053
Anti-LC3B Polyclonal Antibody 2 is a Rabbit antibody targeting LC3B. Anti-LC3B Polyclonal Antibody 2 can be used in WB.
Anti-LC3B Polyclonal Antibody 2
Anti-LC3B Polyclonal Antibody 2
😃Good
Catalog No. TMAB-01053
Anti-LC3B Polyclonal Antibody 2 is a Rabbit antibody targeting LC3B. Anti-LC3B Polyclonal Antibody 2 can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Mouse Testis tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Cerebellum tissue lysates Lane 6: Rat Testis tissue lysates Primary: Anti-LC3B (TMAB-01053) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 15,17 kDa |
| Application | WB |
| Recommended Dose | WB: 1:200-1000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. |
| Immunogen | KLH conjugated synthetic peptide: mouse LC3B |
| Antigen Species | Mouse |
| Gene ID | 67443 |
| Uniprot ID | |
| Biology Area | APG gene products,Signal Transduction,Autophagy,APG gene products,Autophagy and mitophagy,APG gene products,Neurogenesis,MAP |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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