Shopping Cart
Remove All
Your shopping cart is currently empty
Anti-LC3B Polyclonal Antibody (Synonyms: microtubule-associated protein 1 light chain 3 β, microtubule-associat...)
Catalog No. TMAB-01052 Copy Product Info
🥰Excellent
Anti-LC3B Polyclonal Antibody is a Rabbit antibody targeting LC3B. Anti-LC3B Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
Anti-LC3B Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-01052
Synonyms microtubule-associated protein 1 light chain 3 β, microtubule-associated protein 1 light chain 3 beta, MAP1LC3B-a, MAP1A/1BLC3, LC3B, ATG8F
Anti-LC3B Polyclonal Antibody is a Rabbit antibody targeting LC3B. Anti-LC3B Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P.
Anti-LC3B Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
Add to Cart
Add to Quotation
For research use only—not for human use. No sales to individuals. Use as intended only.
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-LC3B Polyclonal Antibody is a Rabbit antibody targeting LC3B. Anti-LC3B Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P. |
| Synonyms | microtubule-associated protein 1 light chain 3 β, microtubule-associated protein 1 light chain 3 beta, MAP1LC3B-a, MAP1A/1BLC3, LC3B, ATG8F |
| Ig Type | IgG |
| Reactivity | Mouse,Rat,Rabbit (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Zebrafish) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (TMAB-01052) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (TMAB-01052) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (LC3B) Polyclonal Antibody, Unconjugated (TMAB-01052) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 4. Sample: Lane 1: Recombinant human LC3B protein, N-His at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13 kDa Observed band size: 15 kDa     |
| Application | |
| Recommended Dose | IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. |
| Tissue Specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. |
| Immunogen | KLH conjugated synthetic peptide: human LC3A/B |
| Antigen Species | Human |
| Gene Name | MAP1LC3B |
| Gene ID | |
| Protein Name | Microtubule-associated proteins 1A/1B light chain 3B |
| Uniprot ID | |
| Biology Area | APG gene products,Signal Transduction,Autophagy,APG gene products,Autophagy and mitophagy,APG gene products,Neurogenesis,MAP |
| Function | Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
| Molecular Weight | Theoretical: 13 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
Preview
Related Tags: buy Anti-LC3B Polyclonal Antibody | purchase Anti-LC3B Polyclonal Antibody | Anti-LC3B Polyclonal Antibody cost | order Anti-LC3B Polyclonal Antibody | Anti-LC3B Polyclonal Antibody molecular weight
Generate Quote
Catalog No.: Cas No.:
Add
| Size | Quantity | Unit Price | Amount | Operation |
|---|
Generate Quote
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2026 TargetMol Chemicals Inc. All Rights Reserved.